Heavy metal (HM) contamination is a persistent environmental problem in many countries. Pesticides, organic compounds, paints, industrial and mining waste, and heavy metals (HMs) are the primary drivers of soil pollution. It is critical to clear up soils contaminated by HMs. It is beneficial to investigate the areas of HM bioremediation employing hybrid technologies, genetically engineered microorganisms with improved bioremediation potential techniques. In the present study three abundantly growing indigenous microbial isolates (Bacteria- Rhizobium and Azotobacter and Fungi- Aspergillus) were cultured using suitable growth media to obtain large biomass. These indigenous species have ability to grow in mass quantity under ideal conditions at minimal efforts. The isolates were then tested for their tolerance to heavy metals Cr, Hg, Cu, Zn, and Mg under experimental settings using MIC and the broth dilution technique at various concentrations (100,300 and 500ppm for bacteria and additionally 1000,3000 and 5000ppm for fungi). All the three isolates showed various degree of tolerance for selected heavy metals. Aspergillus fumigatus responded best among all.
Cite this article:
Shubhangi Jha, Pragya Kulkarni and Anamika Sharma (2023). Heavy Metal Tolerance and Toxicity Studies on- Indigenous Microflora and its Application for Bioremediation. Spectrum of Emerging Sciences, 2(2), pp. 10-16. 10.55878/SES2022-2-2-3DOI: https://doi.org/10.55878/SES2022-2-2-3
AM, Dopp E, Obe G, Rettenmeier AW. Genotoxicity of organometallic species. In:
Hirner AV, Emons H, editors. Organic Metal and Metalloid Species in the
Environment [Internet]. Berlin, Heidelberg: Springer Berlin Heidelberg; 2004
A, Rajasekar A, Theerthagiri J, Ananthaselvam A, Sathishkumar K, Madhavan J, et
al. Integrated Remediation Processes Toward Heavy Metal Removal/Recovery From
Various Environments-A Review. Front Environ Sci. 2019 May 22;7:66.
P, Sharma A, Gangola S, Kumar R, Bhatt P, Kumar G. Impact of Agri-Usable
Nanocompounds on Soil Microbial Activity: An Indicator of Soil Health: Soil.
CLEAN - Soil Air Water. 2017 May;45(5):1600458.
JJ, Kim YS, Kumar V. Heavy metal toxicity: An update of chelating therapeutic
strategies. J Trace Elem Med Biol. 2019 Jul;54:226–31.
S, Kaushik G, Dar MA, Nimesh S, López-Chuken UJ, Villarreal-Chiu JF. Microbial
Degradation of Organophosphate Pesticides: A Review. Pedosphere. 2018
PC, Lee KD, Sreekanth TVM. Heavy metals, occurrence and toxicity for plants: a
review. Environ Chem Lett. 2010 Sep;8(3):199–216.
Y, Luan Y, Ning Y, Wang L. Effects and Mechanisms of Microbial Remediation of
Heavy Metals in Soil: A Critical Review. Appl Sci. 2018 Aug 10;8(8):1336.
| Heavy Metal Removal by Bioaccumulation Using Genetically Engineered
M, Hallajisani A, Keshtkar AR, Shahbeig H, Ali Ghorbanian S. Equilibrium and
kinetic study and modeling of Cu(II) and Co(II) synergistic biosorption from
Cu(II)-Co(II) single and binary mixtures on brown algae C. indica. J Environ
Chem Eng. 2015 Mar;3(1):140–9.
A, Saez JM, Davila Costa JS, Colin VL, Fuentes MS, Cuozzo SA, et al.
Actinobacteria: Current research and perspectives for bioremediation of
pesticides and heavy metals. Chemosphere. 2017 Jan;166:41–62.
C, Franco AR, Pereira SIA, Henriques I, Correia A, Magan N, et al.
Metal(loid)-Contaminated Soils as a Source of Culturable Heterotrophic Aerobic
Bacteria for Remediation Applications. Geomicrobiol J. 2017 Oct 21;34(9):760–8.
Response to Soil Liming of Damaged Ecosystems Revealed by Pyrosequencing and
Phospholipid Fatty Acid Analyses.
Gupta and Pragya Kulkarni. A comparative study on Nostoc and Oscillatoria spp.
for heavy metal tolerance and biomass production. National Journal of Life Science, Vol. 13(2) 2016 : 147-150.
K, Wang Q, Lv M, Chen L. Microorganism remediation strategies towards heavy
metals. Chem Eng J. 2019 Mar; 360:1553–63.